Posts in Assisted Breeding
Genomic Selection in CWRS and CWAD Wheat Breeding

The proposed strategy in this project is to apply genomic selection as early as possible within the breeding programs that is cost effective based on the current cost of genotyping and number of lines under consideration. Our strategy to continue to apply genomic selection at the F4 generation is based on a pilot study discussed more in the next section of this application ‘how does this project build on your previous research or research of others’. By applying genomic selection at an early generation the gene pool of breeding populations will be enriched for desirable alleles in the same way that phenotypic selection is done for agronomic and disease traits such as height and rust resistance.

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Introgressing Thinopyrum intermedium stripe rust resistance genes into wheat

Breeding for biotic stress like stripe rust is an on-going challenge due to the continuous emergence of newly adapted races. The proposed research aims to translate the research findings of a recently concluded upstream research project where novel genetic sources of resistance against pathogens including stripe rust have been identified using the novel RenSeq method, in a diversity panel of intermediate wheatgrass accessions.

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Maximizing durable disease resistance in wheat

The goal of this project is to address disease resistance breeding in wheat by expanding on the novel genetic and genomic resources that has been established by the research team in CTAG and 4D Wheat projects, co funded by Sask Wheat. One of the objectives is to localize new genes for stripe rust resistance in bread wheat lines that are absent in the commercial spring wheat cultivars and to use the marker assisted selection and accelerated backcrossing to transfer Yr15 into Canadian bread and durum wheat varieties.

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Breeding hardier crops for Saskatchewan - Dynamic phenotyping to dissect component of water stress in wheat

The main objectives of this project are to establish a high throughput phenotyping system, to analyze variation in Nested Association Mapping population founder lines of wheat to identify the genotypes better able to transpire and continue photosynthesizing under water stress, to map the underlying quantitative variation controlling biomass accumulation under water stress and to evaluate the stress resilient material in field trails comparing performance under irrigated and non-irrigated conditions.

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Unraveling the genetic mechanisms underlying ergot resistance in durum wheat

Developing durum wheat varieties that are less susceptible to ergot has become a rising priority. With the goal of enhancing the level of understanding of genetic resistance for ergot in the breeding germplasm, this study will include extensive multi-year phenotyping and next generation genotyping of genetic populations, strategic parental selection and crossing, gene stacking and molecular phenotyping using the Canadian Light Source platform.

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Unraveling and stacking of grain protein genes in durum to keep pace with yield increases through breeding

This project focuses on understanding the genes involved in high yield and high protein in Canadian germplasm such as AAC GoldNet, a recently registered cultivar with simultaneous increase in yield and protein. The objective is to map and identify high grain protein content QTL and verify QTL that are “keeping pace” with grain yield improvement using double haploid populations.

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Marker assisted breeding for common bunt resistance in new wheat varieties adapted to the Canadian Prairies

Common bunt is a grading factor in CWRS wheat and listed as a priority 1 disease in the registration system. Down grading of infested grain results in a loss in revenue to farmers. Thus, this project focuses on efforts to boost bunt resistance by gene stacking in CWRS wheat, an approach used by plant breeders to assemble target genes of interest from different sources into breeding lines.

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